Research Article
Production of peptide antibiotics by Bacillus sp. GU 057 indigenously isolated from saline soil
Adnan Amin, Muhammad Ayaz Khan, Malik Ehsanullah, Uzma Haroon, Sheikh Muhammad Farooq Azam, Abdul Hameed
Published:
DOI:
10.1590/S1517-838220120004000015
License:
© Sociedade Brasileira de Microbiologia2012All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
Abstract
A total of 112 soil samples were taken from differents areas of district D.I.Khan and Kohat (KPK) Pakistan and screened for production of antibiotics against the Micrococcus luteus and Staphylococcus aureus. Widest zone of inhibition (18mm) was produced by microorganism isolated from saline soil. The strain was later identified as Bacillus GU057 by standard biochemical assays. Maximum activity (18mm inhibition zone) was observed against Staphylococcus aureus after 48 hours of incubation at pH 8 and 4% concentration of glucose. The antibiotic was identified by autobiography as bacitracin. The Bacillus strain GU057 was confirmed as good peptide antibiotic producer and can effectively be indulged as biocontrol agent.