Research Article
Quantification of maltol in Korean ginseng (Panax ginseng) products by high-performance liquid chromatography-diode array detector
Hyun Cheol Jeong, Hee-Do Hong, Young-Chan Kim, Young Kyoung Rhee, Sang Yoon Choi, Kyung-Tack Kim, Sung Soo Kim, Young-Chul Lee, Chang-Won Cho
Published:
DOI:
10.4103/0973-1296.160452
License:
Copyright: © Pharmacognosy Magazine2015This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background:Maltol, as a type of phenolic compounds, is produced by the browning reaction during the high-temperature treatment of ginseng. Thus, maltol can be used as a marker for the quality control of various ginseng products manufactured by high-temperature treatment including red ginseng. For the quantification of maltol in Korean ginseng products, an effective high-performance liquid chromatography-diode array detector (HPLC-DAD) method was developed.Materials and Methods:The HPLC-DAD method for maltol quantification coupled with a liquid-liquid extraction (LLE) method was developed and validated in terms of linearity, precision, and accuracy. An HPLC separation was performed on a C18 column.Results:The LLE methods and HPLC running conditions for maltol quantification were optimized. The calibration curve of the maltol exhibited good linearity (R2 = 1.00). The limit of detection value of maltol was 0.26 μg/mL, and the limit of quantification value was 0.79 μg/mL. The relative standard deviations (RSDs) of the data of the intra- and inter-day experiments were <1.27% and 0.61%, respectively. The results of the recovery test were 101.35–101.75% with an RSD value of 0.21–1.65%. The developed method was applied successfully to quantify the maltol in three ginseng products manufactured by different methods.Conclusion:The results of validation demonstrated that the proposed HPLC-DAD method was useful for the quantification of maltol in various ginseng products.