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Inducible nitric oxide synthase in the placenta of pregnant women with preeclampsia


Abstract

Introduction. During pregnancy, changes in blood pressure is a major cause of maternal and fetal death worldwide, 12% of all pregnancies develop preeclampsia (PEC). The oxide nitric synthase endothelial enzyme (eNOS) generates nitric oxide continuously, producing vasodilation. The inducible isoform (iNOS) is found in macrophages, smooth muscle cells and endothelial cells, and other locations as platelets, hepatocytes, tumor cells and lymphocytes. The main objective of this work was to detect iNOS by fluorescein in placental tissue.

Methodology. Childbirth after were performed 1X1X0.5cm cuts on the surface of the cotyledons, were placed in 10% formalin were embedded in paraffin for microtome cuts and fix them on lamella. Were subsequently washed with buffer solution, oxygenated water with methanol, using also pig serum 10% (Rockland®, D305, lot # 421), solution-RabbitpAb antiiNOS (Calbioquem®482728), streptavidin-fluorescein (Calbioquem®, Cat. No.189734). Fluorescence microscope Carl Zeiss brand was used, and the Image-Pro Plus, 7.0 software.

Results. Nineteen placentas of pregnant women with PEC were collected and 19 without the pathology.  It find increased uptake of fluorescein in erythrocytes in the maternal placentas no PEC, the patients who developed PEC was remarkable decreased catchment of fluorescein by state of gravity.

Conclusions. There is an obvious decrease in activity of iNOS during preeclampsia, which is a possible explanatory factor on the process of vasoconstriction during pregnancy, manifested by elevated blood pressure.

Poster
non-peer-reviewed

Inducible nitric oxide synthase in the placenta of pregnant women with preeclampsia


Author Information

Juan Carlos Medrano Rodríguez Corresponding Author

Doctorate in Sciences in the specialty of Medical and Molecular Pharmacology, Autonomous University of Zacatecas

Eduardo Medrano Cortés

Autonomous University of Zacatecas, Academic Unit of Dentistry

Christian Starlight Franco Trejo

Autonomous University of Zacatecas, Academic Unit of Dentistry

Hilda Saray Contreras de la Fuente

Autonomous University of Zacatecas, Academic Unit of Nursing


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  • Author Information
    Juan Carlos Medrano Rodríguez Corresponding Author

    Doctorate in Sciences in the specialty of Medical and Molecular Pharmacology, Autonomous University of Zacatecas

    Eduardo Medrano Cortés

    Autonomous University of Zacatecas, Academic Unit of Dentistry

    Christian Starlight Franco Trejo

    Autonomous University of Zacatecas, Academic Unit of Dentistry

    Hilda Saray Contreras de la Fuente

    Autonomous University of Zacatecas, Academic Unit of Nursing

    Poster Information
    Meeting

    First International Online BioMedical Conference September 09, 2015 - September 12, 2015

    Publication history

    Received by Cureus: August 12, 2015
    Published: September 09, 2015

    License

    This is an open access poster distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Introduction. During pregnancy, changes in blood pressure is a major cause of maternal and fetal death worldwide, 12% of all pregnancies develop preeclampsia (PEC). The oxide nitric synthase endothelial enzyme (eNOS) generates nitric oxide continuously, producing vasodilation. The inducible isoform (iNOS) is found in macrophages, smooth muscle cells and endothelial cells, and other locations as platelets, hepatocytes, tumor cells and lymphocytes. The main objective of this work was to detect iNOS by fluorescein in placental tissue.

Methodology. Childbirth after were performed 1X1X0.5cm cuts on the surface of the cotyledons, were placed in 10% formalin were embedded in paraffin for microtome cuts and fix them on lamella. Were subsequently washed with buffer solution, oxygenated water with methanol, using also pig serum 10% (Rockland®, D305, lot # 421), solution-RabbitpAb antiiNOS (Calbioquem®482728), streptavidin-fluorescein (Calbioquem®, Cat. No.189734). Fluorescence microscope Carl Zeiss brand was used, and the Image-Pro Plus, 7.0 software.

Results. Nineteen placentas of pregnant women with PEC were collected and 19 without the pathology.  It find increased uptake of fluorescein in erythrocytes in the maternal placentas no PEC, the patients who developed PEC was remarkable decreased catchment of fluorescein by state of gravity.

Conclusions. There is an obvious decrease in activity of iNOS during preeclampsia, which is a possible explanatory factor on the process of vasoconstriction during pregnancy, manifested by elevated blood pressure.

Juan Carlos Medrano Rodríguez, Ph.D.

Doctorate in Sciences in the specialty of Medical and Molecular Pharmacology, Autonomous University of Zacatecas

For correspondence:
merodi12@hotmail.com

Eduardo Medrano Cortés

Autonomous University of Zacatecas, Academic Unit of Dentistry

Christian Starlight Franco Trejo

Autonomous University of Zacatecas, Academic Unit of Dentistry

Hilda Saray Contreras de la Fuente

Autonomous University of Zacatecas, Academic Unit of Nursing

Juan Carlos Medrano Rodríguez, Ph.D.

Doctorate in Sciences in the specialty of Medical and Molecular Pharmacology, Autonomous University of Zacatecas

For correspondence:
merodi12@hotmail.com

Eduardo Medrano Cortés

Autonomous University of Zacatecas, Academic Unit of Dentistry

Christian Starlight Franco Trejo

Autonomous University of Zacatecas, Academic Unit of Dentistry

Hilda Saray Contreras de la Fuente

Autonomous University of Zacatecas, Academic Unit of Nursing

Juan Carlos Medrano Rodríguez, Ph.D.

Doctorate in Sciences in the specialty of Medical and Molecular Pharmacology, Autonomous University of Zacatecas

For correspondence:
merodi12@hotmail.com

Eduardo Medrano Cortés

Autonomous University of Zacatecas, Academic Unit of Dentistry

Christian Starlight Franco Trejo

Autonomous University of Zacatecas, Academic Unit of Dentistry

Hilda Saray Contreras de la Fuente

Autonomous University of Zacatecas, Academic Unit of Nursing

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