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Technical report
peer-reviewed

Laboratory Diagnosis of Scabies Using a Simple Saline Mount: A Clinical Microbiologist's Report



Abstract

Scabies is a skin condition caused by infestation of the mite Sarcoptes scabiei. Sarcoptes scabiei var. hominis is an arthropod member belonging to the order Acarina. Scabies is present throughout the world and is prevalent in all age groups, mostly involving people frequently in contact with animals, children, women, and elderly people. Predisposing factors for scabies include individuals with immunosuppressive conditions and people residing under low socioeconomic conditions. Skin lesions that occur during mite infestation closely resemble dermatological disorders caused by microbes, including fungi, parasites, and viruses. Laboratory diagnosis of scabies greatly relies on an accurate clinical suspicion and the demonstration of mites in the skin scrapings could be used for confirmation. It should be noted that a greater understanding between a clinician or a dermatologist and a clinical microbiologist is required to successfully diagnose scabies. This report details an easily performed, cost-effective method, the simple saline mount, that a clinical microbiology laboratory should follow to successfully identify mites in skin scrapings.



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Technical report
peer-reviewed

Laboratory Diagnosis of Scabies Using a Simple Saline Mount: A Clinical Microbiologist's Report


Author Information

Venkataramana Kandi Corresponding Author

Department of Microbiology, Prathima Institute of Medical Sciences


Ethics Statement and Conflict of Interest Disclosures

Human subjects: Consent was obtained by all participants in this study. Prathima Institute of Medical Sciences-IEC issued approval PIMS/IEC/2017-100210. Animal subjects: This study did not involve animal subjects or tissue. Conflicts of interest: The authors have declared that no conflicts of interest exist.


Technical report
peer-reviewed

Laboratory Diagnosis of Scabies Using a Simple Saline Mount: A Clinical Microbiologist's Report


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Technical report
peer-reviewed

Laboratory Diagnosis of Scabies Using a Simple Saline Mount: A Clinical Microbiologist's Report

  • Author Information
    Venkataramana Kandi Corresponding Author

    Department of Microbiology, Prathima Institute of Medical Sciences


    Ethics Statement and Conflict of Interest Disclosures

    Human subjects: Consent was obtained by all participants in this study. Prathima Institute of Medical Sciences-IEC issued approval PIMS/IEC/2017-100210. Animal subjects: This study did not involve animal subjects or tissue. Conflicts of interest: The authors have declared that no conflicts of interest exist.

    Acknowledgements


    Article Information

    Published: March 19, 2017

    DOI

    10.7759/cureus.1102

    Cite this article as:

    Kandi V (March 19, 2017) Laboratory Diagnosis of Scabies Using a Simple Saline Mount: A Clinical Microbiologist's Report. Cureus 9(3): e1102. doi:10.7759/cureus.1102

    Publication history

    Received by Cureus: March 05, 2017
    Peer review began: March 10, 2017
    Peer review concluded: March 14, 2017
    Published: March 19, 2017

    Copyright

    © Copyright 2017
    Kandi. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 3.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    License

    This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Scabies is a skin condition caused by infestation of the mite Sarcoptes scabiei. Sarcoptes scabiei var. hominis is an arthropod member belonging to the order Acarina. Scabies is present throughout the world and is prevalent in all age groups, mostly involving people frequently in contact with animals, children, women, and elderly people. Predisposing factors for scabies include individuals with immunosuppressive conditions and people residing under low socioeconomic conditions. Skin lesions that occur during mite infestation closely resemble dermatological disorders caused by microbes, including fungi, parasites, and viruses. Laboratory diagnosis of scabies greatly relies on an accurate clinical suspicion and the demonstration of mites in the skin scrapings could be used for confirmation. It should be noted that a greater understanding between a clinician or a dermatologist and a clinical microbiologist is required to successfully diagnose scabies. This report details an easily performed, cost-effective method, the simple saline mount, that a clinical microbiology laboratory should follow to successfully identify mites in skin scrapings.



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Venkataramana Kandi, Ph.D.

Department of Microbiology, Prathima Institute of Medical Sciences

For correspondence:
ramana20021@gmail.com

Venkataramana Kandi, Ph.D.

Department of Microbiology, Prathima Institute of Medical Sciences

For correspondence:
ramana20021@gmail.com