"Never doubt that a small group of thoughtful, committed citizens can change the world. Indeed, it is the only thing that ever has."

Margaret Mead

Original article
peer-reviewed

The Frequency and Risk Factors Identified for HBsAg and Anti-HCV in a Preoperative Screening of Patients Admitted in the Surgical Department at Al-Tibri Medical College Hospital



Abstract

Objective

The aim of this study is to determine the frequency and risk factors for hepatitis B surface antigen (HBsAg) and anti-hepatitis C virus (Anti-HCV) in a preoperative screening of patients admitted at the surgical unit of Al-Tibri Medical College and Hospital and Lyari General Hospital, Karachi.

Materials and methods

This study was conducted at the surgical units of Al-Tibri Medical College Hospital and Lyari General Hospital, Karachi. This study was conducted for six months from 30th June 2017 to 31st December 2017. This observational study was performed using a non-probability convenient sampling technique. All patients who were undergoing elective and emergency surgical procedure were selected for the study and screened for HBsAg and anti-HCV by immunochromatographic test (ICT) method at the hospital laboratory. The risk factors identified as parenteral injections, past surgical procedure, blood transfusion, etc. along with demographic data as age, gender, and district residential status were recorded on a specially designed proforma. Data were analyzed using Statistical Package for the Social Sciences (SPSS) version 23. Descriptive statistics were applied, and the qualitative data were expressed as the mean and standard deviation.

Results

Among a total of 360 patients included in the study, 63 (21.38%) were found with anti-HCV positive, and 14 (3.88%) were HBsAg positive with none of the patients found to have co-infection. The male-to-female ratio was 2.3:1. The mean age of patients was 32.34±4.3 years. The age range affected commonly in the study population was 21-30 years, 24(31%). The commonest risk factor for transmission of viral infection was parenteral injection abuser 31(40.2%), followed by surgical procedure 13(18.1%), blood transfusion 10(12.9%), and barber shave 7(9.1%).

Conclusion

It was predicted in our study that hepatitis C was more common in patients screened preoperatively for surgery. However, the most frequent risk factor in these patients was parenteral abuse history. The preoperative screening is an important investigative tool for the identification of silent cases in the community for preventive measures adoption and treatment for silent carriers.
 

Introduction

Hepatitis, by definition, is inflammation of the liver, which if untreated or undiagnosed causes shrinkage of the liver, leading to functional devastating consequences on the human body. Hepatitis B and C are the leading causes of liver diseases, and the World Health Organization (WHO) estimates a 3% prevalence of hepatitis C virus (HCV) worldwide [1]. The annual mortality owing to hepatitis B virus (HBV)-related acute and chronic liver disease is estimated to be 5.2 million. In a developing country like Pakistan, poverty and lack of resources and social awareness with dangerous, unhealthy practices account for the enormous burden of chronic Hepatitis B and C-related liver diseases. The prevalence of Hepatitis B is 10% and is 7% for Hepatitis C [2].

The major causative factors of chronic liver disease are hepatitis B and C, with both being the most common public health issues in society [3]. Recent records reveal that 175 million people have hepatitis C virus. Globally, around 500,000 to 1000,000 HBV-induced mortalities have been noticed yearly owing to hepatocellular carcinoma and cirrhosis [4]. The Centre for Disease Control and Prevention of the United States of America concludes that 2.7 million population is suffering from hepatitis C-induced comorbidities, affecting 40% of patients with chronic liver diseases; these alarming statistics focus on the increased need for liver transplantation [5-6].

Hepatitis B virus (HBV) is frequently found among children and young Asian population [7]. In Pakistan, HBV is found in the middle zone with an incidence rate of 3 to 4%. However, HCV and HBV have become a prominent health issue [8]. In general, all the age group populations are affected, as the potential risk factors are widespread with ignorance and poverty, especially in the rural areas in our country, as our study population primarily includes rural dwellers of poor socioeconomic class. This study is an addition to the local research studies in Pakistan conducted to identify silent seropositive patients on preoperative screen; over the years, up to 25% increase has been observed in the semi-urban population [9]. The most significant risk factors are injurious use of unsterilized syringes, history of blood transfusion, and surgical procedures [10]. The prevalence of Hepatitis C is as high as 20.8% in a healthy and high-risk population, sharing syringes as evident in a study conducted at Quetta in apparently healthy blood donors [11]

The emergence of seropositive patients is a serious community health issue, and identification is important in preoperative time to follow strict safety precautions in theatre for prevention of spread. The objective to carry out the study at teaching hospitals was to emphasize preoperative screening in minor and major cases with strict theatre precautions and directed for further treatment, as silent cases as the first study of such type at both teaching institutions. This study aimed to screen in pre-operative time patients for hepatitis B surface antigen (HBsAg) and anti-HCV positive by immunochromatographic test (ICT) method along with risk factors for the disease.

Materials & Methods

This cross-sectional descriptive study using a non-probability purposive sampling technique was conducted in the surgical department of Al-Tibri Medical College and Hospital and Lyari General Hospital, Karachi. This study was conducted for six months, from 30th June 2017 to 31st December 2017. The ethical approval for the study was taken from the Institutional Research and Ethical committee. Verbal informed consent was taken from the patients or their guardians.

A total of 360 patients were selected during the study period, and they were admitted for elective and emergency surgical procedures. All patients aged >5 years, who are not known to be anti-HCV and HBsAg seropositive previously, were included in the study. Chronic carriers or patients on antiviral treatment were excluded from the study. All the cases were preoperatively screened for HBsAg and anti-HCV. Hepatitis B virus and HCV screening are very common and mandatory in tertiary care hospitals as a pre-operative investigative tool for the assessment of their incidence as well as to adapt well preventive measures before surgery. Serology was performed and evaluated by following standard operating procedures. Rapid ICT for qualitative assessment of hepatitis B surface antigen and detection of antibodies for hepatitis C was performed as a screening technique in the study. The cases that were reactive to the rapid method kit were further reassessed using enzyme-linked immunosorbent (ELISA). The cases which were found positive on ELISA were included in the study. The risk factors identified on specially designed proforma were parenteral injections, past blood transfusion, barber shave, and surgical procedures. Proforma for data collection was designed and kept in the ward, and doctors were assigned as part of the research, who collected data for risk factors and documented. The data collected were analyzed using the Statistical Package for Social Sciences (SPSS) Version 23. The quantitative data were reported as the frequency in percentages, and results were generated.

Results

A total of 360 patients were selected for the study by strictly following the inclusion criteria. All of these subjects were screened for anti-HCV and HBsAg by ICT. Out of these, 63 patients were anti-HCV positive and 14 were HBsAg positive (Table 1), while none of the patients had co-infections. The mean age of patients was 42.42±7.58 years with a male-to-female ratio was 2.3:1.

ICT Testing*  n (%) 
HBsAg+  14 (3.88%) 
Anti-HCV ++ 63 (21.38%) 
Negative  283 (74.74%) 
Total   360 (100%) 

The patients were categorized based on five age groups, and their frequency and percentage were reported. The groups included age groups between 21-30, 31-40 years, 41-50 years, >50 years, and <20 years. The mean age of patients was 32.34±4.3 years. The commonest frequency observed was in the age group of 21-30 years for both viruses in 24 (31.1%) cases. The minimum frequency was found in the last age group of <20 years with 9 (11.6%) cases having only HCV (Table 2). 

Variable  HBsAg+ n (%)  Anti HCV++ n (%) 
Age groups   <20 Years  0 (0.0%)  9 (14.28%) 
21-30 Years  7 (50.0%)  17 (26.98%) 
31-40 Years  5 (35.7%)  14 (22.22%) 
41-50 Years  2 (14.29%)  13 (20.63%) 
>50 Years   0 (0.0%)  11 (17.46%) 
Total  14 (18.8%)  63 (81.8%) 

Data regarding risk factors were recorded as follows: 10 (12.9%) cases had a history of blood transfusion, 13 (18.1%) patients had past surgical procedures, 31 (40.2%) subjects had an addiction of parenteral abuse, 7 (9.1%) cases had a history of barber shave, 5 (6.4%) cases had parenteral abuse as well as past surgical procedure history, 6 (7.3%) cases had a history of barber shave and past surgical events, and 5 (6%) subjects had a history of past dental surgery (Figure 1).

Discussion

Viral hepatitis cases, in both acute and chronic carrier forms, are increasing in number, worldwide [12]. The prevalence of Hepatitis B and C-associated liver diseases is higher in the underdeveloped world due to the lack of awareness and immature health practices, contrary to the industrialized western world; the low burden of disease is mainly due to immigrants and chronic carriers [13]. Hepatitis B carrier rate in Pakistan is 3-4%, and 10 million Pakistani populations are Hepatitis C carriers. In this study, the frequency and male-to-female ratio are comparable to a local study done by Rafique et al. [2].

Our study found more male patients (53.2%), compared to females (46.8%), being infected with the virus, as validated by the Eastern literature, which shows that males from the rural and lower socioeconomic classes were seropositive for Hepatitis C [14]. Our study population comprised patients from the rural area of district Gadap and poor urban dwellers of district Lyari. The Western literature has less quantitative data on Hepatitis B and C-related health issues; however, the emerging immigrant populations from endemic zones such as Asia and Africa-owing to the insurgency, terrorism, and asylum to the west-have contributed to the increased prevalence of affected males in Canada, from 0.8% to 1%, as suggested by Sonal et al. [15].

In our study, the frequency for Hepatitis C and Hepatitis B was found to be 21.3% and 3.8%, indicating a fourfold increase for Hepatitis C. This could be probably due to the Expanded Program on Immunisation (EPI), administrating Hepatitis B vaccine in newborns providing some protection against virus transmission and lifelong immunity. However, unhealthy practice and lack of awareness are contributing significantly to the increase in Hepatitis C, as the virus has a long silent carrier state detected incidentally on preoperative screening by Sonal A et al. [13,15]. In the present study, the age group of 21-30 years is more commonly affected comparable with a study by Kausar et al. in pregnant females although males are more affected in our study [10]. However, Mahesh et al. found HBsAg and anti-HCV in the male population of age group 40-50 years predominantly, in contrast to our study where 20-30 years age group has the highest infection rate [12]. This variation could be in qualitative sample selection as cataract patients were included in their study, whereas all cases of age range from 5 to 65 years in our study. The incidence of hepatitis B can be prevented using vaccine, and hence an awareness should be raised in society for those who come for surgery and test negative so that they can be prevented and those who have encountered it should be offered treatment.

Our study population simulates a study by Ayesha et al. that found a 20.8% prevalence of HCV virus in young, healthy male blood donors [3]. This preoperative screening with comparable results by Paryal et al. is alarming, and cheap, rapid screening in preoperative time for all surgical procedures at private and public sector institutes should be recommended for enhanced safety precautions and directional treatment for carriers [11,13]. The commonest risk factor in our study was found to be parenteral injection (40.2%) followed by past surgical procedures, comparable to the local study by Rafique et al. as well as the study by Achakzai et al. involving healthy and susceptible individuals [2,16]. Also, Khatoon et al. study based on the rural population identified a similar frequency of risk factors at a rural hospital in India [14].

Our suggestion for this major health issue is in agreement with a study from Eastern Europe by Ganczak et al. for making screening policy in preoperative time for blood-borne viral transmission of the disease by rapid kit test or ELISA, to curtail risk factors in the community and treat the silent carriers [17]. Due to the lack of resources, preoperative screening was done by rapid kit test (ICT); seropositive patients were directed for ELISA and further treatment, although Ijaz et al. found no statistical difference in different ICT kit results with the gold standard ELISA test conducted for HBsAg and anti-HCV [18]. Therefore, this study might not be immune to practice bias.

Conclusions

HCV was found to be the most common virus in preoperatively screened surgery patients in our study. However, parenteral abuse was found to be the most frequent risk factor in these surgical patients. The age group that tested mostly positive for HCV was young patients (21-30 years). Perhaps, for the prevention of these silent cases in the community that are detected while undergoing workup for surgery, screening is an important investigative tool that can help implement the treatment of these carrier cases.


References

  1. Paryal T, Mujeeb R, Rafique P: Hepatitis B & C;Frequency of viral infections in surgical patients. Prof Med J. 2017, 24:278-81. 10.17957/TPMJ/17.3592
  2. Rafique M, Altaf S, Saima A: Frequency of Hepatitis B and C in patients undergoing elective surgery. J Ayub Med Coll Abottabad. 2010, 22:167-70.
  3. Strader DB, Wright T, Thomas DL, et al.: Diagnosis, management, and treatment of Hepatitis C. Hepatology. 2004, 39:1147. 10.1002/hep.20119
  4. EASL International Consensus Conference on Hepatitis B.13-14 September 2002. Geneva, Switzerland Consensus statement (short version). J Hepatol. 2003, 38:533-540. 10.1016/s0168-8278(03)00083-7
  5. Dienstag JL, McHutchison JG: American Gastroenterological Association Technical review on management of hepatitis C. Gastroenterology. 2006, 130:231-264. 10.1053/j.gastro.2005.11.010
  6. Lauer GM, Walker BD: Hepatitis C virus infection. NEJM. 2001, 345:41-52. 10.1056/NEJM200107053450107
  7. Aziz S, Muzaffar R, Hafiz S, et al.: Helicobacter pylori, Hepatitis viruses A, C, E antibodies and HBsAg - Prevalence and associated risk factors in pediatric community of Karachi. JCPSP. 2007, 17:195-198. 04.2007/jcpsp.195198
  8. Alam MS, Zaidi SZ, Malik SA, et al.: Serology based disease status of Pakistani population infected with Hepatitis B virus. BMC Infect dis. 2007, 7:2334-64.
  9. Ayesha K, Abdul Malik TA, Aamer I: Prevalence of HCV among the young male blood donors of Quetta region of Balochistan Pakistan. Virology J. 2013, 10:1-4.
  10. Kausar J, Bushra Z, Qadir B: Frequency and the risk factors of Hepatitis C virus in pregnant women; A hospital based descriptive study in Gadap Town Karachi. Pak J Med Sci. 2017, 33:1265-67. 10.12669/pjms.335.12493
  11. Saad N, Efaza U, Abdul Nafey K: Prevalence of Hepatitis B and C among preoperative cataract patients in Karachi. BMC Res N. 2012, 5:1-4.
  12. Mahesh KL, Li Su, Nasrani AK, et al.: Frequency of Hepatitis B surface antigen (HBsAg) and Hepatitis C antibody (HCVAb) seropositivity among preoperative eye surgery patients. BJMP. 2016, 9:918.
  13. Huda W, Jameel N, Rehman Fu: Prevalence of Hepatitis B and C in urban patients undergoing cataract surgery. Pak J Ophth. 2013, 29:147-50.
  14. Khatoon R, Jahan N: Assessment of seroprevalance of Hepatitis C virus specific antibodies among patients attending hospital of semi urban North India using rapid qualitative in vitro diagnostic test trop. Ann Trop Med Public Health. 2017, 10:199.
  15. Sonal A, Norman K: Operating on a patient with Hepatitis C. Can J Surg. 2009, 52:337-42.
  16. Achakazai M, Kassi M: Seroprevalance and coinfection of HIV, Hepatitis C and B virus in injecting drug users in Quetta Pakistan. Trop Doct. 2007, 37:43-45.
  17. GanczakM, Szych Z: Rationale for the implementation of preoperative testing for HCV in the light of anti-HCV and HBsAg test results in surgical patients from a teaching hospital. Przegl Epidemiol. 2009, 63:387-92.
  18. Ijaz M, Asad M, Khalid A: Comparison of different ICT Kits for HbsAg and ANTI HCV using gold standard by ELISA. Pak J Med. 2012, 51:72-76.
Original article
peer-reviewed

The Frequency and Risk Factors Identified for HBsAg and Anti-HCV in a Preoperative Screening of Patients Admitted in the Surgical Department at Al-Tibri Medical College Hospital


Author Information

Sadaf Jabeen Malik

Surgery, Al-Tibri Medical College, Isra University, Karachi, PAK

Rekha Melwani

Surgery, Al-Tibri Medical College, Isra University, Karachi, PAK

Farhat Bano

Surgery, Shaheed Muhtarama Benazir Bhutto Medical College, Karachi, PAK

Bilal Suria

Urology, Al-Tibri Medical College and Hospital, Isra University, Karachi, PAK

Ihsanullah Sial

Surgery, Al-Tibri Medical College and Hospital, Isra University, Karachi, PAK

Bushra Tasneem

Surgery, Abbasi Shaheed Hospital, Karachi, PAK

Madiha Ariff Corresponding Author

Internal Medicine, Dow University of Health Sciences, Karachi, PAK


Ethics Statement and Conflict of Interest Disclosures

Human subjects: Consent was obtained by all participants in this study. Al-Tibri Medical College Hospital issued approval 6783456. This research is safe to be conducted. All patient demographic should be kept confidential. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.


Original article
peer-reviewed

The Frequency and Risk Factors Identified for HBsAg and Anti-HCV in a Preoperative Screening of Patients Admitted in the Surgical Department at Al-Tibri Medical College Hospital


Figures etc.

SIQ
3.9
RATED BY 2 READERS
CONTRIBUTE RATING

Scholary Impact Quotient™ (SIQ™) is our unique post-publication peer review rating process. Learn more here.