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Case report
peer-reviewed

Invasive Lobular Carcinoma with Extracellular Mucin Production: Description of a Case and Review of the Literature



Abstract

Invasive lobular carcinoma of the breast is the second most common subtype of breast carcinoma. It accounts for 5-15% of the breast carcinoma cases reported. It shows a different metastatic pattern compared to invasive carcinoma of no special type. There are several variants of this cancer including the classic, solid, signet ring cell, tubulolobular, alveolar, trabecular, pleomorphic, and mixed subtypes each one with a distinctive morphology. Invasive lobular carcinoma has been associated with intracellular mucin production, in the form of intracytoplasmic lumina and signet ring cells whereas extracellular mucin production has been related to carcinomas of ductal origin. A new variant that displays extracellular mucin production was described recently. Only nineteen cases of this rare entity have been reported to date. In this manuscript, we report an additional case of invasive lobular carcinoma with extracellular mucin production, describing its clinico-pathological characteristics, and review the literature on the same.

Introduction

Invasive lobular carcinoma (ILC) of the breast is the second most common variant of breast carcinoma. It accounts for 5-15% of invasive mammary carcinomas [1]. It usually affects older patients. It is more often bilateral and multifocal or multicentric compared to invasive carcinoma of no special type. Also, the metastatic pattern of ILC is different, showing a tendency to metastasize to the gastrointestinal tract, meninges, bone and the female genital tract [1].

Histologically, the classic variant of ILC is characterized by distinct morphology that consists of small cells lacking cohesion arranged in a single file pattern, and sometimes in a concentric pattern around existing ducts and lobular units [2]. Tumor cells have intra-cytoplasmic lumina, and a variable number of signet ring cells (SRC) are present. The mitotic count is low. Several different histological variants have been described including the solid, signet ring cell, tubulolobular, alveolar, trabecular, pleomorphic, and mixed variants. Stromal mucin is present in several benign or malignant breast lesions. Traditionally extracellular mucin production is associated with carcinomas of ductal origin. Furthermore, some authors consider mucinous cystadenocarcinoma of the breast to originate from mucinous metaplasia [3].

In 2009 Rosa et al. [4] described a new variant of ILC that displayed extracellular mucin production after which 20 cases have been reported in the English literature [2, 5-11]. In this manuscript, we report an additional case of ILC with extracellular mucin production. We also review the literature and discuss the differential diagnosis.

Case Presentation

A 65-year-old female patient was admitted to our hospital's surgical department after discovering a small palpable lump on the left upper quadrant of her right breast during self-examination. Upon physical examination, a solid mass with a maximum diameter of around 2cm with an irregular contour was detected upon palpation. Imaging studies were consistent with malignancy. During surgery, frozen sections of the tumor and sentinel lymph node biopsy were performed. The breast lump was positive for malignancy. Sentinel lymph node was negative for metastatic disease. A simple right mastectomy was performed. On gross examination, the tumor was soft, pale, grayish blue, gelatin-like, and well-circumscribed. On microscopic examination, the tumor consisted of two separate foci measuring 13 and 6mm, respectively. Both foci consisted of a non-mucinous ILC component of the classical (figure 1A) and solid variant and a mucinous component. The mucinous component represented about 20% of the tumor. Extracellular mucin production was seen in the form of multiple, relatively circumscribed, nodular areas (figure 1B), and patchy extracellular mucin production with irregular borders (figure 1C). Tumor cells in the mucinous component were arranged in clusters (figure 1D) and single cells. Several signet ring cells were identified in both areas (figure 1E, 1F).

Using Immunohistochemistry, the tumor cells stained positive for estrogen receptors (ER) (figure 2A) and negative for progesterone receptors (PR) (figure 2B), HER-2 (figure 2C), and E-Cadherin (figure 2D). Proliferation index Ki-67 stained 10% of tumor nuclei. Based on morphological and immunohistochemistry results, the tumor was signed out as ILC Grade 2 with extracellular mucin production.

A multidisciplinary tumor board suggested that administration of adjuvant chemotherapy was not appropriate. Thus, radiotherapy and hormonal therapy were offered. Ten months after surgery, the patient is alive without evidence of recurrence or metastasis.

Discussion

ILC with extracellular mucin production is a rare variant of ILC. Our literature review revealed seven articles describing single cases and two case series describing eight and four cases, respectively [2,4-11]. Several articles do not report relevant data, such as lymph node status and treatment. Information concerning follow-up and outcome were published only in one case series [9].

All patients were female. Patients’ age ranged from 38 to 87 years (median age 64). Most patients presented with a palpable mass and/or a mammographic abnormality. Tumor median size was 43 mm (range 8-100 mm). All previous cases comprised two components, a mucinous and a non-mucinous one. The mucinous part consisted either of nodular lakes of mucin or was patchy with irregular borders and represented 10 to 70% of the tumor area. Seven patients presented with a grade 2 tumor, five of which had a grade 3 tumor and only two patients had a grade 1 tumor. In all tumors except for one case in the series reported by Singh et al., variable numbers of SRC were found [10]. Rarely, E-cadherin negative glandular or pseudocribriform formations were observed. Nine out of sixteen cases with available information had lymph node metastasis. In twelve out of nineteen cases, lobular neoplasia was present.

In all cases with available data, ER was positive, PR was positive in ten and negative in four cases, and Her-2 was over-expressed in two, negative in eleven, and was scored 1+ in one and 2+ in two cases. None of these cases were amplified with fluorescent in situ hybridization. Ki67 proliferation index expression ranged from 7 to 40%. The clinico-pathological features of all previous cases are presented in Table 1.

Case Author Year Age Size (mm) Grade ER % PR % HER2 Ki-67 (%)
1 Rosa et al. [4] 2009 60 90 NA NA NA NA NA NA
2 Yu et al. [2] 2010 65 ΝΑ 1+ * NA 100 N 3+ 25
3 Haltas et al. [5] 2012 43 ΝΑ 1/19 NA P P N NA
4 Bari et al. [6] 2015 38 35 2/10 NA P P N NA
5 Gomez-Macias et al. [7] 2016 60 9 0/4 1 P 90 N NA
6 Cserni et al. [9] 2017 69 >24 1/2 2 100 10 N 20
7 Cserni et al. [9] 2017 65 90 11/13 2 90 5 N 30
8 Cserni et al. [9] 2017 71 46 0/3 2 90 40 3+ 10
9 Cserni et al. [9] 2017 62 80 10/23 2 90 80 Namp 40
10 Cserni et al. [9] 2017 45 29 0/2 3 95 95 N 40
11 Cserni et al. [9] 2017 56 22 0/1 2 100 70 N 20
12 Cserni et al. [9] 2017 75 30 7/9  2/3 80 <1 N 20
13 Cserni et al. [9] 2017 60 50 3/13  2 60 5 Namp 7
14 Boukhechba et al. [8] 2018 75 15 NA NA P N N NA
15 Singh et al. [10] 2019 87 100 1/3 2 P NA N NA
16 Singh et al. [10] 2019 72 16 NA 3 P NA N NA
17 Singh et al. [10] 2019 70 >20 0/10 3 P NA N NA
18 Singh et al. [10] 2019 77 8 0/2 1 P NA N NA
19 Baig et al. [11] 2019 67 60 0/2** 3 P N Namp NA
20 Present case 2019 65 13 0/2 2 100 N N 10

All patients underwent surgical treatment. Six patients were treated with breast-conserving surgery (BCS), five of which underwent a mastectomy and one of them was treated with excisional biopsy (EB). Three patients, two treated initially with BCS, and one with EB underwent a mastectomy subsequently. Sentinel lymph node biopsy was performed in six, and axillary lymph node dissection in five patients. Lymph node metastasis was found in nine out of sixteen patients with reported nodal status. Hormonal therapy, chemotherapy, and radiotherapy were administered in five, four, and eight patients, respectively. One patient was treated initially with neoadjuvant therapy. Treatment features are reported in table 2.

Case Author Surgery Adjuvant therapy Follow up (months)
1 Rosa et al. [4] M NA NA
2 Yu et al. [2] BCS + SLNB NA NA
3 Haltas et al. [5] M + ALND NA NA
4 Bari et al. [6] M + ALND NA NA
5 Gomez-Macias et al. [7] BCS + SLNB RT + HT NA
6 Cserni et al. [9] M + SLNB RT + HT 26
7 Cserni et al. [9] BCS > M RT + HT + ChT 40
8 Cserni et al. [9] BCS + SNB RT + HT + ChT 29
9 Cserni et al. [9] BCS > M + ALND RT 68
10 Cserni et al. [9] BCS + SLNB RT + ChT 2
11 Cserni et al. [9] M + SLNB RT 11
12 Cserni et al. [9] EB > M + ALND Neoadj ChT 21
13 Cserni et al. [9] M + ALND RT + HT + ChT NA
14 Boukhechba et al. [8] NA NA NA
15 Singh et al. [10] NA NA NA
16 Singh et al. [10] NA NA NA
17 Singh et al. [10] NA NA NA
18 Singh et al. [10] NA NA NA
19 Baig et al. [11] BCS ChT+RT+HT NA
20 Present case M+ SLNB RT + HT 8

Some of the previously reported cases were initially misdiagnosed as mucinous carcinomas or as invasive carcinomas of no special type with extracellular mucin production. This may be explained by the fact that the initial diagnosis was made in limited biopsy specimens in which only the mucinous area was sampled [11]. The differential diagnosis includes pure mucinous carcinoma, mixed mucinous- no special type carcinoma, solid papillary carcinoma, polymorphous mammary carcinoma [9], and metaplastic matrix-producing carcinoma [12]. Pure mucinous carcinoma lacks the component with the morphological characteristics of ILC. Mixed mucinous-no special type carcinoma or mixed mucinous-ILC may be more diagnostically challenging since two different components are present that may sometimes simulate those found in ILC with extracellular mucin production. Careful examination with attention to morphology will distinguish most if not all cases. Solid papillary carcinoma may occasionally be associated with extracellular mucin production and invasive mucinous carcinoma [9], but it lacks the distinctive morphology of ILC. The mucoid-like stroma of polymorphous mammary adenocarcinoma and matrix-producing carcinoma may be confused with the mucin lakes of ILC with extracellular mucin production. Lack of expression of estrogen and progesterone receptors in both tumors as well as other characteristics will help distinguish them from ILC [12,13]. In all the previously mentioned entities, when facing diagnostic difficulty, an immunohistochemical panel consisting of E-cadherin, b-catenin, and P120 immunostains will give the solution. We believe that the precise diagnosis of this variant of ILC is essential since several of its histological mimics have a different treatment and prognosis.

Conclusions

ILC with extracellular mucin production is a rare variant of ILC with a distinct morphology. Its diagnosis may occasionally be challenging, especially when dealing with limited biopsy specimens. The diagnosis of a mucinous carcinoma should not be made just by examining a tumor on low power. The presence of discohesive growth, bland cytological features, intracytoplasmic lumina, and SRC found on high power examination, should alert the pathologist. The use of appropriate immunohistochemical stains will assist in avoiding a misdiagnosis. Precise diagnosis is the key to correct treatment since most of its histological mimics have different treatment and prognosis.


References

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  2. Yu J, Bhargava R, Dabbs DJ: Invasive lobular carcinoma with extracellular mucin production and HER-2 overexpression: a case report and further case studies. Diagn Pathol. 2010, 5:36. 10.1186/1746-1596-5-36
  3. Koufopoulos N, Goudeli C, Syrios J, Filopoulos E, Khaldi L: Mucinous cystadenocarcinoma of the breast: the challenge of diagnosing a rare entity. Rare tumors. 2017, 9:98-100. 10.4081/rt.2017.7016
  4. Rosa M, Mohammadi A, Masood S: Lobular carcinoma of the breast with extracellular mucin: new variant of mucin-producing carcinomas?. Pathol Int. 2009, 59:405-409. 10.1111/j.1440-1827.2009.02385.x
  5. Haltas H, Bayrak R, Yenidunya S, Kosehan D, Sen M, Akin K: Invasive lobular carcinoma with extracellular mucin as a distinct variant of lobular carcinoma: a case report. Diagn Pathol. 2012, 7:91. 10.1186/1746-1596-7-91
  6. Bari VB, Bholay SU, Sane KC: Invasive lobular carcinoma of the breast with extracellular mucin- a new rare variant. J Clin Diagn Res. 2015, 9:05-06. 10.7860/JCDR/2015/13220.5765
  7. Gomez Macias GS, Perez Saucedo JE, Cardona Huerta S, Garza Montemayor M, Villarreal Garza C, Garcia Hernandez I: Invasive lobular carcinoma of the breast with extracellular mucin: A case report. Int J Surg Case Rep. 2016, 25:33-36. 10.1016/j.ijscr.2016.05.056
  8. Boukhechba M, Kadiri H, El Khannoussi B: Invasive lobular carcinoma of the breast with extracellular mucin: case report of a new variant of lobular carcinoma of the breast. Case Rep Pathol. 2018, 2018:1-3. 10.1155/2018/5362951
  9. Cserni G, Floris G, Koufopoulos N, et al.: Invasive lobular carcinoma with extracellular mucin production-a novel pattern of lobular carcinomas of the breast. Clinico-pathological description of eight cases. Virchows Arch. 2017, 471:3-12. 10.1007/s00428-017-2147-6
  10. Singh K, DiazGomez B, Wang Y, Ou J, Hansen K: Invasive lobular carcinoma with extracellular mucin: not all mucinous mammary carcinomas are ductal! . Int J Surg Pathol. 2019, 27:55-58. 10.1177/1066896918788660
  11. Baig A, Omeroglu-Altinel G, Omeroglu A: Invasive pleomorphic-type lobular carcinoma of the breast presenting as a mucinous carcinoma. Case Rep Pathol. 2019, 2019:5. 10.1155/2019/1839208
  12. Koufopoulos N, Kokkali S, Antoniadou F, Dimas D, Missitzis I: Matrix-producing breast carcinoma: A rare subtype of metaplastic breast carcinoma. Cureus. 2019, 11:5188. 10.7759/cureus.5188
  13. Asioli S, Marucci G, Ficarra G, Stephens M, Foschini MP, Ellis IO, Eusebi V: Polymorphous adenocarcinoma of the breast. Report of three cases. Virchows Arch. 2006, 448:29-34. 10.1007/s00428-005-0084-2
Case report
peer-reviewed

Invasive Lobular Carcinoma with Extracellular Mucin Production: Description of a Case and Review of the Literature


Author Information

Nektarios Koufopoulos Corresponding Author

Pathology, Attikon University Hospital, Medical School of Athens, Athens, GRC

Pathology, Saint Savvas Cancer Hospital, Athens, GRC

Foteini Antoniadou

Pathology, Saint Savvas Cancer Hospital, Athens, GRC

Stefania Kokkali

Oncology, Saint Savvas Cancer Hospital, Athens, GRC

Eleni Pigadioti

Pathology, Metropolitan Hospital of Athens, Athens, GRC

Pathology, Saint Savvas Cancer Hospital, Athens, GRC

Lubna Khaldi

Pathology, Saint Savvas Cancer Hospital, Athens, GRC


Ethics Statement and Conflict of Interest Disclosures

Human subjects: Consent was obtained by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.


Case report
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Invasive Lobular Carcinoma with Extracellular Mucin Production: Description of a Case and Review of the Literature


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