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Original article

Evaluation of the Activities of Thyroid Hormones Among Pre- and Post-menopausal Euthyroid Women: A Cross-sectional Study from a Tertiary Care Teaching Hospital in India



Aging brings about several changes in humans that include both physiological and anatomical changes. As individuals' age, the activity of the thyroid gland and its hormones decline, causing significant metabolic disorders. Most thyroid gland disorders have been noted among young and middle-aged women. Very little is known regarding the activities of thyroid hormones among older aged women.


The study included 350 young to middle-aged pre-menopausal women between 25 and 49 years and 350 older post-menopausal women above 50 years of age. The study was conducted in the department of biochemistry, Prathima Institute of Medical Sciences (PIMS), Nagunur, Karimnagar, Telangana, India. The subjects included in the study were euthyroid (not having any signs and symptoms of thyroid disorder) and were not on any medication. The thyroid profile, including thyroid stimulating hormone (TSH), tri-iodothyronine (T3), and tetra-iodothyronine (T4), was analyzed in all the study subjects using the chemiluminescence immunoassay (CLIA) technique on a completely automated Abbott i1000SR Architect Plus instrument (Abbott Core Laboratory, Illinois‎, US).


There was no statistically significant difference in thyroid hormone activities in the two age groups compared, as noted by the unpaired student's ‘t’ test. The mean serum TSH levels in the older post-menopausal women (3.39+2.45) were found to be higher than those noted in pre-menopausal women (2.60+1.31). The activities of Tand T4 showed no difference in both groups (p=0.8397).


The study results clearly indicate an increase in the activities of TSH among the older-aged post-menopausal women.


Human life relies on a delicate balance of hormones such as estrogen, progesterone, testosterone, and many others. Women come across many physiological and anatomical changes throughout their lives, including puberty, pregnancy, and menopause, which are controlled by female sex hormones. Thyroid hormones influence the development and functioning of the reproductive system and the overall body metabolism.

Thyroid diseases mainly affect women; the incidence is five to 20 times higher in women than in men. The prevalence of thyroid diseases increases with age. In women, diseases of the thyroid gland are among the most prevalent disorders worldwide, second only to diabetes [1].

Thyroid diseases are more common in middle-aged and older post-menopausal women. The diagnosis and interpretation of thyroid function tests, including an estimation of the activities of thyroid stimulating hormone (TSH), tri-iodothyronine (T3), and tetra-iodothyronine (T4) in old-age people are very difficult [2].

Most professional organizations recommend the screening of older women for thyroid dysfunction. The American Thyroid Association (ATA), the Endocrine Society and the American Association of Clinical Endocrinologists (AACE) had recommended aggressive case finding in elderly women [3]. According to the 2012 clinical practice guidelines formulated by the AACE and ATA, serum TSH is the best screening test for the primary diagnosis of thyroid disorders [3]. TSH is the preferred test to assess thyroid function, as stated by the National Academy of Clinical Biochemistry (NACB) [4].

Thyroid functions can be influenced by nutritional status, associated co-morbidities, co-factors such as body surface area, and others [5-6]. A regular follow-up of the activities of TSH could help in the clinical diagnosis and better management of the patients. According to the available literature, serum TSH concentrations may probably be age-dependent. Increased levels of serum TSH in the elderly, mainly in women, can be physiological or pathological [7-8].

This comparative cross-sectional study was undertaken to measure the serum TSH, T3, and T4 activities in young, middle-aged pre-menopausal women and post-menopausal older-aged women.

Materials & Methods

This prospective study included 350 young and middle-aged pre-menopausal women (20-49 years) and 350 elderly post-menopausal women (>50 years). All the subjects enrolled in the study were female patients attending the outpatient department of medicine at Prathima Institute of Medical Sciences (PIMS), Karimnagar, Telangana, India. Informed and oral consent was obtained from all the study participants and the study was approved by the institutional ethical committee.

All the subjects included in the study had no clinical symptoms of thyroid disorder and were not on any medication. Patients with a history of major chronic illnesses, e.g. diabetes mellitus, hypertension, other endocrinal disorders, patients on hormone replacement therapy or on drug altering serum TSH, hysterectomy patients, pre-pubertal age women, pregnant woman, and premature menopausal women were excluded from the study.

Five milliliters of venous blood was drawn aseptically from all the study subjects and serum TSH, T3, and Twere measured by using chemiluminescence/magnetic particle immunoassay (CLIA) using an automated analyzer (Abbott i1000SR Architect Plus). The serum concentrations of TSH, T3, and T4 were expressed in μIU/ml and interpreted according to the manufacturer's recommendations.


Post-menopausal women showed slightly increased TSH activity (3.39±2.45) as compared to pre-menopausal women (2.60±1.31). The activities of T3 and T4, however, revealed no variation among the study groups. Table 1 shows the serum activities of TSH, T3, and T4 among both study groups.

Thyroid hormones Pre-menopausal women Post-menopausal women Normal range p-value
(Mean±SD) (Mean±SD)
TSH 2.60±1.31 3.39±2.45 0.35-4.94 µIU/mL 0.097
T3 1.03±0.24 0.99±0.19 0.64-1.52 ng/mL 0.551
T4 7.81±1.29 7.88±1.26 4.87-11.72 µg/dL 0.839


In the present study, we encountered mean serum TSH activities in older post-menopausal women (3.39±2.45 µIU/mL) that were comparatively higher than those observed among young, middle-aged pre-menopausal women (2.60±1.35 µIU/mL). However, the difference was not statistically significant. Multiple causes were proposed for increased TSH activities in the elderly, including nutritional iodine supply, sleep disturbances, altered sleep patterns, and others [9]. Aging is associated with changes in the pituitary-thyroid axis and there was a progressive shift in the serum TSH activities with increasing age [10]. The endocrine system undergoes changes with aging even in the absence of overt disease. Thus, an age-related fall in T4 activities and the reduced responsiveness of the thyroid to TSH could result in increased TSH secretion [11].

Another possibility of increased TSH activities may be due to occult thyroid disease in older people [12]. With aging, the variations in the activities of serum TSH may not be too significant as compared to the activities of other endocrine hormones, such as the ones secreted by the adrenal gland, as noted in previous studies [13].

Rojas LV et al. found higher TSH activities in post-menopausal women (2.80 µIU/mL) as compared to pre-menopausal (2.52 µIU/mL). They found an average increase in TSH values with age, although the change between groups was not significant, which was similar to the results observed from the current study. It has been suggested that an evaluation of baseline TSH levels within a group and in a defined geographical location may be significant [14].

A large, population-based study conducted by the National Health And Nutrition Examination Survey (NHANES) found higher TSH activities in women in the older age group [15]. An analysis of NHANES III (2007) showed that age-related shifts in TSH activities were not significantly altered when individuals with antithyroid antibodies were excluded from the study. Alterations in the activities of thyroid hormones under the influence of food (soy) were also previously reported [16]. Aging could influence the activities of various hormones that include the growth hormone, growth hormone-releasing hormone (GHRH), estrogen, progesterone, androgens, follicle-stimulating hormone, insulin-like growth factor 1, and others [17]. Therefore, it is important to understand the activities of thyroid hormones with aging.

Other previous studies showed completely contrasting results, where the serum TSH activities among the post-menopausal and older women were lower as compared to the pre-menopausal women who had increased activities of serum TSH [18-20]. These studies have suggested that this type of scenario may be age-related and could have been influenced by the pituitary gland.

Not many previous studies have evaluated the activities of thyroid hormones among euthyroid women. A recent study from the USA had evaluated the relationship between the thyroid hormonal activities and the menstrual function outcomes among the euthyroid women. This study had noted that the thyroid hormonal activities were influencing the menstrual function outcomes and the sex steroid hormonal activities, suggesting its potential relation to fertility [21].

Another recent research from India reported a study that included 301 euthyroid, pre-, and post-menopausal women suffering from type 2 diabetes. This study selected non-obese, obese, and overweight type 2 diabetes patients and evaluated their thyroid hormonal activities. The study observed a statistically significant (p<0.01) relation of the activities of T4 and TSH within the pre and post-menopausal women [22].

The evaluation of the activities of thyroid hormones among the pre-menopausal women was suggested in a previous study. This study had correlated the possibility of early atherosclerosis and the activities of TSH among the euthyroid women with autoimmune thyroiditis. This study had suggested that although euthyroid, there is a possibility of developing cardiovascular disease with the increased activities of TSH [23].

Probable mechanisms and contributing factors of thyroid dysfunction 

As evidenced by the current literature, there is a possibility of variation in the activities of thyroid hormones among women. Experimental studies in the past have highlighted the potential role of estrogen in the development of thyroid dysfunction. Studies found that estrogen receptors, along with their isoforms, on thyroid cells could modulate thyroid function, especially causing cancer [24-25].

The most accepted and probably the potential mechanism by which estrogen causes thyroid dysfunction among women, especially post-menopausal women, is its binding to the thyroglobulin. This restricts the entry of thyroxine into the cells, thereby increasing the concentrations of bound thyroxine and reducing the availability of free thyroid hormones [26].

Thyroid disease among the post-menopausal women was also attributed to the environmental toxicity caused by an increase in polybrominated diphenyl ethers (PBDEs), as evidenced by a study by Allen JG et al. from the United States of America (USA) [27].

Thyroid disease in the elderly was also attributed to autoimmunity, drugs, radiotherapy, and surgery. The activities of TSH among the elderly were found to be influenced by drugs that include glucocorticoids, somatostatin analogs, dopamine agonists, metformin, antiepileptics, lithium carbonate, and iodine-containing drugs. Other drugs, such as antacids, amiodarone, estrogens, mitotane, fluorouracil, phenobarbital, and rifampin, were also found to cause thyroid dysfunction [28]. Chronic exposure to bisphenol A (BPA), an organic compound used in making plastic, was also found to cause endocrinal abnormalities affecting human health [29]. Although its role in causing thyroid dysfunction is not yet confirmed, future studies on the potential role of BPA in causing thyroid diseases appear to be important, in view of the extensive use of plastics in developing countries like India.

Thyroid disease was also attributed to the reactivation of Epstein-Barr virus (EBV) in a recent study from Japan. This study had noted that during EBV reactivation, thyrotropin receptor antibodies (TRAbs) are formed, which in turn stimulates the TSH receptors and causes thyroid dysfunction [30].


In view of the results obtained from the current research and considering the previous reports, there is a possibility of a shift in the activities of thyroid hormones with age. Considering the improved life expectancy and the fact that the activities of thyroid hormones could influence the reproductive and other metabolic pathways, it is important for us to have a better understanding of the activities of thyroid hormones as a person ages for appropriate management. Also, there is a need to understand the baseline thyroid function by measuring the activities of thyroid hormones in euthyroid women at various climacteric stages of life, including pre-puberty, after puberty, pre-pregnancy, after pregnancy, and post-menopause to determine subclinical thyroid disease/thyroid dysfunction.


  1. World Health Organization: Diagnosis and monitoring of diseases of the thyroid. (2000). Accessed: December 15, 2018: https://apps.who.int/iris/bitstream/handle/10665/66342/WHO_DIL_00.4_eng.pdf?sequence=1&isAllowed=y.
  2. Schindler AE: Thyroid function and postmenopause. Gynecol Endocrinol. 2003, 17:79-85. 10.1080/gye.
  3. Garber JR, Cobin RH, Gharib H, et al.: Clinical practice guidelines for hypothyroidism in adults: cosponsored by the American Association of Clinical Endocrinologists and the American Thyroid Association. Endocr Pract. 2012, 18:988-1028. 10.4158/EP12280.GL
  4. Demers LM, Spencer CA: Laboratory medicine practice guidelines: laboratory support for the diagnosis and monitoring of thyroid disease. Clin Endocrinol (Oxf). 2003, 58:138-140. 10.1046/j.1365-2265.2003.01681.x
  5. Holthausen FF, von Müller F, Happel C, Kranert WT, Grünwald F: Age and body composition influence TSH concentrations after administration of rhTSH [Article in German]. Nuklearmedizin. 2015, 54:20-25. 10.3413/Nukmed-0673-14-06
  6. Guastamacchia E, Giagulli VA, Licchelli B, Triggiani V: Selenium and Iodine in autoimmune thyroiditis. Endocr Metab Immune Disord Drug Targets. 2015, 15:288-292. 10.2174/1871530315666150619094242
  7. Dunn D, Turner C: Hypothyroidism in women. Nurs Womens Health. 2016, 20:93-98. 10.1016/j.nwh.2015.12.002
  8. Geng H, Zhang X, Wang C, et al.: Even mildly elevated TSH is associated with an atherogenic lipid profile in postmenopausal women with subclinical hypothyroidism. Endocr Res. 2015, 40:1-7. 10.3109/07435800.2013.879166
  9. Sarne D: Effects of the environment, chemicals and drugs on thyroid function. Endotext [Internet]. De Groot LJ, Beck-Peccoz P, Chrousos G, Dungan K, Grossman A, Hershman JM, Koch C, McLachlan R, New M, Rebar R, Singer F, Vinik A, Weickert MO (ed): MDText.com, Inc., South Dartmouth, MA; 2000. 2000:NBK285560.
  10. Benhadi N, Fliers E, Visser TJ, Reitsma JB, Wiersinga WM: Pilot study on the assessment of the setpoint of the hypothalamus-pituitary-thyroid axis in healthy volunteers. Eur J Endocrinol. 2010, 162:323-329. 10.1530/EJE-09-0655
  11. Brabant G, Prank K, Ranft U, et al.: Physiological regulation of circadian and pulsatile thyrotropin secretion in normal man and woman. J Clin Endocrinol Metab. 1990, 70:403-409. 10.1210/jcem-70-2-403
  12. Surks MI, Hollowell JG: Age-specific distribution of serum thyrotropin and antithyroid antibodies in the US population: implications for the prevalence of subclinical hypothyroidism. J Clin Endocrinol Metab. 2007, 92:4575-4582. 10.1210/jc.2007-1499
  13. Chahal HS, Drake WM: The endocrine system and ageing. J Pathol. 2007, 211:173-180. 10.1002/path.2110
  14. Rojas LV, Nieves K, Suarez E, Ortiz AP, Rivera A, Romaguera J: Thyroid-stimulating hormone and follicle-stimulating hormone status in Hispanic women during the menopause transition. Ethn Dis. 2008, 18:230-234.
  15. Hollowell JG, Staehling NW, Flanders WD, Hannon WH, Gunter EW, Spencer CA, Braverman LE: Serum TSH, T(4), and thyroid antibodies in the United States population (1988 to 1994): National Health and Nutrition Examination Survey (NHANES III). J Clin Endocrinol Metab. 2002, 87:489-499. 10.1210/jcem.87.2.8182
  16. Sathyapalan T, Köhrle J, Rijntjes E, Rigby AS, Dargham SR, Kilpatrick ES, Atkin SL: The effect of high dose isoflavone supplementation on serum reverse T3 in euthyroid men with type 2 diabetes and post-menopausal women. Front Endocrinol (Lausanne). 2018, 9:698. 10.3389/fendo.2018.00698
  17. Kuokkanen S, Santoro N: Endocrinology of the perimenopausal woman. Glob Libr Women's Med. 2011, 2011:Accessed: February 15, 2018: https://www.glowm.com/section_view/heading/Endocrinology%20of%20the%20Perimenopausal%20Woman/item/82. 10.3843/GLOWM.10082
  18. Pearce EN: Thyroid dysfunction in perimenopausal and postmenopausal women. Menopause Int. 2007, 13:8-13. 10.1258/175404507780456746
  19. Mariotti S, Barbesino G, Caturegli P, et al.: Complex alteration of thyroid function in healthy centenarians. J Clin Endocrinol Metab. 1993, 77:1133-1134. 10.1210/jcem.77.5.8077303
  20. Hershman JM, Pekary AE, Berg L, Solomon DH, Sawin CT: Serum thyrotropin and thyroid hormone levels in elderly and middle-aged euthyroid persons. J Am Geriatr Soc. 1993, 41:823-828. 10.1111/j.1532-5415.1993.tb06177.x
  21. Jacobson MH, Howards PP, Darrow LA, et al.: Thyroid hormones and menstrual cycle function in a longitudinal cohort of premenopausal women. Paediatr Perinat Epidemiol. 2018, 32:225-234. 10.1111/ppe.12462
  22. Jayanthi R, Srinivasan AR, Niranjan G: Thyroid status in premenopausal and postmenopausal women - a biochemical study on insulin resistance in non obese, overweight and obese type 2 diabetics. Diabetes Metab Syndr. 2018, 12:859-862. 10.1016/j.dsx.2018.05.001
  23. Topaloglu O, Gokay F, Kucukler K, et al.: Is autoimmune thyroiditis a risk factor for early atherosclerosis in premenopausal women even if in euthyroid status?. Endocrine. 2013, 44:145-151. 10.1007/s12020-012-9842-5
  24. Santin AP, Furlanetto TW: Role of estrogen in thyroid function and growth regulation. Thyroid Res. 2011, 2011:875125. 10.4061/2011/875125
  25. Xu S, Chen G, Peng W, Renko K, Derwahl M: Oestrogen action on thyroid progenitor cells: relevant for the pathogenesis of thyroid nodules?. J Endocrinol. 2013, 218:125-133. 10.1530/JOE-13-0029
  26. Mazer NA: Interaction of estrogen therapy and thyroid hormone replacement in postmenopausal women. Thyroid. 2004, 14:S27-34. 10.1089/105072504323024561
  27. Allen JG, Gale S, Zoeller RT, Spengler JD, Birnbaum L, McNeely E: PBDE flame retardants, thyroid disease, and menopausal status in U.S. women. Environ Health. 2016, 15:60. 10.1186/s12940-016-0141-0
  28. Faggiano A, Del Prete M, Marciello F, Marotta V, Ramundo V, Colao A: Thyroid diseases in elderly. Minerva Endocrinol. 2011, 36:211-231.
  29. Rochester JR: Bisphenol A and human health: a review of the literature. Reprod Toxicol. 2013, 42:132-155. 10.1016/j.reprotox.2013.08.008
  30. Hara S, Nagata K, Kumata K, et al.: Estradiol affects Epstein-Barr virus reactivation-induced thyrotropin receptor antibody and immunoglobulin production in Graves' disease patients and healthy controls. Viral Immunol. 2018, 31:486-491. 10.1089/vim.2018.0032

Original article

Evaluation of the Activities of Thyroid Hormones Among Pre- and Post-menopausal Euthyroid Women: A Cross-sectional Study from a Tertiary Care Teaching Hospital in India

Author Information

Bhagavan Reddy Kolanu

Biochemistry, Prathima Institute of Medical Sciences, Karimnagar, IND

Sabitha Vadakedath

Biochemistry, Chalmeda Anandrao Institute of Medical Sciences, Karimnagar, IND

Venugopal Boddula

Biochemistry, Prathima Institute of Medical Sciences, Karimnagar, IND

Venkataramana Kandi Corresponding Author

Microbiology, Prathima Institute of Medical Sciences, Karimnagar, IND

Ethics Statement and Conflict of Interest Disclosures

Human subjects: Consent was obtained by all participants in this study. Institutional Ethical Committee of Prathima Institute of Medical Sciences issued approval PIMS/IEC/2018-111. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.

Original article

Evaluation of the Activities of Thyroid Hormones Among Pre- and Post-menopausal Euthyroid Women: A Cross-sectional Study from a Tertiary Care Teaching Hospital in India

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