I have completed my Master of Science in Biochemistry in 2017 and presently working in the King George Medical University (KGMU) Lucknow, India as a research staff. During my basic education, I was a bit focused on completing the education with the goal of having a good score and was keen to pursue my scientific career in interdisciplinary research, I gained knowledge in different areas like Cancer Biology, Molecular Biology, Cell Biology and Clinical Sciences. At present, I am working on the novel biomarker based clinical and molecular study in Osteosarcoma subjects. This project is funded by KGMUand about to complete this year.In my current research Iam involving with some Clinical research and working on the identifying the biomarker on Osteosarcoma subjects.I’m having a goodhands-on experience to work withCell culture, Enzyme assays, spectrophotometric analysis, quantitative and qualitative analysis of carbohydrate and amino acids,Western blotting,Real Time PCR, Cell cycle analysis using Flow Cytometry and trained in Nanoporesequencing, MALDI and HPLC.
Previously, I was involved in a study to identify the role of Bisphenol A (BPA; a well-known endocrine disruptor) in epithelial to mesenchymal transition and its implication in breast cancer metastasis. BPA is known to alter the plasticity of breast tissue and is implicated in breast carcinogenesis via endocrine disruption. Various in vitro, in vivo and epidemiological studies, have shown the correlation of BPA with breast carcinogenesis. However, the role of BPA in epithelial to mesenchymal transitions ispoorly known. Repeated exposure of BPA in MCF-7 cancer cells showed a change in the epithelial to mesenchymal transition. Moreover, a significant increase in the ER expression was noted in MCF-7 cells under similar treatment conditions.