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Original article
peer-reviewed

Comparison of a Fully Synthetic Electrospun Matrix to a Bi-Layered Xenograft in Healing Full Thickness Cutaneous Wounds in a Porcine Model



Abstract

A fully synthetic electrospun matrix was compared to a bi-layered xenograft in the healing of full thickness cutaneous wounds in Yucatan miniature swine. Full thickness wounds were created along the dorsum, to which these matrices were applied. The wound area was measured over the course of healing and wound tissue was scored for evidence of inflammation and healing. Animals were sacrificed at Day 15 and Day 30 and tissue samples from the wound site were harvested for histopathological analysis to evaluate inflammation and tissue healing as evidenced by granulation tissue, collagen maturation, vascularization, and epithelialization. Average wound area was significantly smaller for treatment group wounds compared to control group wounds at 15 and 30 days ([7.7 cm2 ± 0.9]/[3.8 cm2 ± 0.8]) and ([2.9 cm2 ± 1.1]/[0.2 cm2 ± 0.0]) (control/treatment) (p = 0.002/p = 0.01). Histopathological analysis of wound sections revealed superior quality of healing with treatment group wounds, as measured by inflammatory response, granulation tissue, and re-epithelialization. A fully synthetic electrospun matrix was associated with faster rates of wound closure characterized by granulation tissue, deposition of mature collagen and vascularization at earlier time points than in wounds treated with a bi-layered xenograft. Treatment with this fully synthetic material may represent a new standard of care by facilitating full-thickness wound closure while eliminating the risks of inflammatory response and disease transmission associated with biologic modalities.



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Original article
peer-reviewed

Comparison of a Fully Synthetic Electrospun Matrix to a Bi-Layered Xenograft in Healing Full Thickness Cutaneous Wounds in a Porcine Model


Author Information

Matthew R. MacEwan

Research & Development, Acera Surgical, Inc

Sarah MacEwan

Research & Development, Acera Surgical, Inc

Anna P. Wright

Research & Development, Acera Surgical, Inc

Tamas R. Kovacs

Research & Development, Acera Surgical, Inc

Joel Batts Corresponding Author

Research, Telos Partners, Llc

Luke Zhang

Research, Sinclair Research Center


Ethics Statement and Conflict of Interest Disclosures

Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: Sinclair Research Center, Animal Care and Use Committee Issued protocol number D14119. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: This study was funded by Acera Surgical, Inc. Financial relationships: Matthew MacEwan, Tamas Kovacs declare(s) stock/stock options from Acera Surgical, Inc. Matthew MacEwan, Anna Wright, Tamas Kovacs declare(s) employment from Acera Surgical, Inc. Sarah MacEwan, Joel Batts, Luke Zhang declare(s) personal fees from Acera Surgical, Inc. Joel Batts declare(s) personal fees from Avita Medical. Intellectual property info: The Restrata matrix examined in this study is currently patent pending under US Application number 20130197663 A1. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.


Original article
peer-reviewed

Comparison of a Fully Synthetic Electrospun Matrix to a Bi-Layered Xenograft in Healing Full Thickness Cutaneous Wounds in a Porcine Model


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Original article
peer-reviewed

Comparison of a Fully Synthetic Electrospun Matrix to a Bi-Layered Xenograft in Healing Full Thickness Cutaneous Wounds in a Porcine Model

Matthew R. MacEwan">Matthew R. MacEwan, Sarah MacEwan ">Sarah MacEwan , Anna P. Wright">Anna P. Wright, Tamas R. Kovacs">Tamas R. Kovacs, Joel Batts">Joel Batts , Luke Zhang">Luke Zhang

  • Author Information
    Matthew R. MacEwan

    Research & Development, Acera Surgical, Inc

    Sarah MacEwan

    Research & Development, Acera Surgical, Inc

    Anna P. Wright

    Research & Development, Acera Surgical, Inc

    Tamas R. Kovacs

    Research & Development, Acera Surgical, Inc

    Joel Batts Corresponding Author

    Research, Telos Partners, Llc

    Luke Zhang

    Research, Sinclair Research Center


    Ethics Statement and Conflict of Interest Disclosures

    Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: Sinclair Research Center, Animal Care and Use Committee Issued protocol number D14119. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: This study was funded by Acera Surgical, Inc. Financial relationships: Matthew MacEwan, Tamas Kovacs declare(s) stock/stock options from Acera Surgical, Inc. Matthew MacEwan, Anna Wright, Tamas Kovacs declare(s) employment from Acera Surgical, Inc. Sarah MacEwan, Joel Batts, Luke Zhang declare(s) personal fees from Acera Surgical, Inc. Joel Batts declare(s) personal fees from Avita Medical. Intellectual property info: The Restrata matrix examined in this study is currently patent pending under US Application number 20130197663 A1. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.

    Acknowledgements


    Article Information

    Published: August 27, 2017

    DOI

    10.7759/cureus.1614

    Cite this article as:

    Macewan M R, Macewan S, Wright A P, et al. (August 27, 2017) Comparison of a Fully Synthetic Electrospun Matrix to a Bi-Layered Xenograft in Healing Full Thickness Cutaneous Wounds in a Porcine Model. Cureus 9(8): e1614. doi:10.7759/cureus.1614

    Publication history

    Received by Cureus: June 30, 2017
    Peer review began: July 26, 2017
    Peer review concluded: August 22, 2017
    Published: August 27, 2017

    Copyright

    © Copyright 2017
    MacEwan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 3.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    License

    This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A fully synthetic electrospun matrix was compared to a bi-layered xenograft in the healing of full thickness cutaneous wounds in Yucatan miniature swine. Full thickness wounds were created along the dorsum, to which these matrices were applied. The wound area was measured over the course of healing and wound tissue was scored for evidence of inflammation and healing. Animals were sacrificed at Day 15 and Day 30 and tissue samples from the wound site were harvested for histopathological analysis to evaluate inflammation and tissue healing as evidenced by granulation tissue, collagen maturation, vascularization, and epithelialization. Average wound area was significantly smaller for treatment group wounds compared to control group wounds at 15 and 30 days ([7.7 cm2 ± 0.9]/[3.8 cm2 ± 0.8]) and ([2.9 cm2 ± 1.1]/[0.2 cm2 ± 0.0]) (control/treatment) (p = 0.002/p = 0.01). Histopathological analysis of wound sections revealed superior quality of healing with treatment group wounds, as measured by inflammatory response, granulation tissue, and re-epithelialization. A fully synthetic electrospun matrix was associated with faster rates of wound closure characterized by granulation tissue, deposition of mature collagen and vascularization at earlier time points than in wounds treated with a bi-layered xenograft. Treatment with this fully synthetic material may represent a new standard of care by facilitating full-thickness wound closure while eliminating the risks of inflammatory response and disease transmission associated with biologic modalities.



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Matthew R. MacEwan

Research & Development, Acera Surgical, Inc

Sarah MacEwan

Research & Development, Acera Surgical, Inc

Anna P. Wright

Research & Development, Acera Surgical, Inc

Tamas R. Kovacs

Research & Development, Acera Surgical, Inc

Joel Batts, MA, Bioethics and Health Policy

Research, Telos Partners, Llc

For correspondence:
jbatts@telospartnersllc.com

Luke Zhang

Research, Sinclair Research Center

Matthew R. MacEwan

Research & Development, Acera Surgical, Inc

Sarah MacEwan

Research & Development, Acera Surgical, Inc

Anna P. Wright

Research & Development, Acera Surgical, Inc

Tamas R. Kovacs

Research & Development, Acera Surgical, Inc

Joel Batts, MA, Bioethics and Health Policy

Research, Telos Partners, Llc

For correspondence:
jbatts@telospartnersllc.com

Luke Zhang

Research, Sinclair Research Center