Chronic Periodontitis is Associated With Cerebral Atherosclerosis –A Nationwide Study

Introduction Chronic periodontitis and atherosclerosis share common risk factors and produce the same inflammatory markers. Many studies found a high prevalence of chronic periodontitis in patients with atherosclerosis but there is no strong evidence to support a specific association of chronic periodontitis with cerebral atherosclerosis. We aimed to study the concurrent prevalence and association of chronic periodontitis with cerebral atherosclerosis and cerebrovascular diseases among the US population. Methods We performed a cross-sectional analysis of a Nationwide Inpatient Sample with adult hospitalizations to identify the primary diagnosis of cerebrovascular diseases [acute ischemic stroke (AIS), hemorrhagic stroke (HS), and transient ischemic attack (TIA)] with concurrent cerebral atherosclerosis and chronic periodontitis. Multivariate survey logistic regression models were fitted to evaluate the linkage of chronic periodontitis with cerebral atherosclerosis and cerebrovascular diseases. Results Of total 56,499,788 hospitalizations, 0.01% had chronic periodontitis. Prevalence of chronic periodontitis was higher in 50-64 years (36.18% vs. 23.91%), males (59.19% vs. 41.06% in females), Afro-Americans (25.93% vs. 15.21%), and 0-25th percentile median-household-income-category (38.31% vs. 30.15%) compared to non-chronic periodontitis. There was significantly higher prevalence of cerebral atherosclerosis (0.71% vs. 0.41%; p<0.0001) with weak evidence of high prevalence of cerebrovascular diseases (AIS:2.21% vs. 1.97%; p=0.1563; HS:0.57% vs. 0.46%; p=0.1560) among chronic periodontitis compared to non-chronic periodontitis. In regression analysis, odds of having cerebral atherosclerosis were 2.48-folds higher in patients with chronic periodontitis compared to that without-chronic periodontitis, and cerebral atherosclerosis patients were associated with higher odds of TIA (aOR:2.40; p<0.0001), AIS (aOR:3.35; p<0.0001), and HS (aOR:1.51; p<0.0001) compared to without-cerebral atherosclerosis. No significant relationship between chronic periodontitis and cerebrovascular diseases was observed. Conclusion Although chronic periodontitis may not directly increase the risk of cerebrovascular diseases, it increases the burden of cerebrovascular diseases by evidently increasing the risk of cerebral atherosclerosis. Early identification of chronic periodontitis and atherosclerotic risk factors may help to mitigate the risk of cerebrovascular diseases.


Introduction
Chronic periodontitis is a chronic inflammation caused by bacterial colonization that affects the periodontal tissue supporting the teeth. The prevalence of chronic periodontitis is high, affecting 90% of the global population and therefore contributes significantly to the global burden of chronic diseases [1,2]. Hence, chronic periodontitis is arising as a major public health issue. According to a recent report from the Centers for Disease Control and Prevention (CDC), 47.2% of adults >30 years of age have periodontitis which increases with age and 70.1% of people by >65 years of age have chronic periodontitis [3].
Chronic Periodontitis can be a risk factor for many systemic diseases like cardiovascular disease, carotid artery disease, cerebrovascular disease, etc. contributing significantly to mortality and morbidity worldwide due to common inflammatory pathogenesis [1,4,5]. Studies have shown that chronic periodontitis patients are prone to the dissemination of oral bacteria and endotoxins into the bloodstream, which can trigger a pro-atherogenic response in endothelial cells leading to systemic inflammation and fibrin deposition. Eventually, this increased systemic inflammatory response is associated with the onset and progression of atherosclerosis and cerebrovascular disease [6][7][8][9]. Chronic periodontitis shares many concurrent conditions and risk factors for vascular disorders like atherosclerosis, stroke, cardiovascular disease, hypertension, dyslipidemia, diabetes mellitus, and smoking [10].
The exact prevalence of periodontitis in a patient with cerebral atherosclerosis and cerebrovascular diseases is not known. Few meta-analyses including both retrospective and prospective studies have reported a higher risk of cardio-and cerebrovascular disease in patients with chronic periodontitis [5,[11][12][13][14]. But subtypes of stroke such as acute ischemic stroke (AIS), hemorrhagic stroke (HS), and transient ischemic attack (TIA) have not been analyzed separately in those meta-analyses. Also, those meta-analyses were from small cohort observational studies, and the estimated association may not be true considering the influence of biases (selection and observer) and confounding factors. All these limitations with previous studies prompt the need for a large population-based study to determine the prevalence of chronic periodontitis in a large group of patients with cerebral atherosclerosis and cerebrovascular diseases.
In this cross-sectional retrospective study, we aimed to describe the prevalence of cerebral atherosclerosis and cerebrovascular diseases among a large cohort of patients with concurrent chronic periodontitis and to assess whether chronic periodontitis is associated with cerebral atherosclerosis and cerebrovascular diseases like AIS, HS, and TIA.

Materials And Methods
We have obtained Nationwide Inpatient Sample (NIS) data from the Healthcare Cost and Utilization Project (HCUP) between January 2013 and December 2014. The NIS is the all-payer inpatient care database in the US and contains discharge-level data provided by states that participate in the HCUP. This dataset acquires approximately 20% of the stratified sample of all US community hospitals, representing more than 95% of the national population. Each hospitalization is treated as an individual entry in the database and is coded with one principal diagnosis and up to 24 secondary diagnoses associated with that stay. Detailed information on NIS is available at http://www.hcup-us.ahrq.gov/db/nation/nis/nisdde.jsp. The NIS is a deidentified database, so informed consent or IRB approval was not needed for the study. The HCUP Data Use Agreement (HCUP-348L73IZS) for the data utilized in this study was obtained. [15]

Outcomes
The primary aim of the study is to identify the prevalence of cerebral atherosclerosis and cerebrovascular diseases (TIA, AIS, and HS) among patients with concurrent chronic periodontitis. The secondary aim is to evaluate the association of cerebral atherosclerosis and cerebrovascular diseases with chronic periodontitis.

Statistical analysis
All statistical analyses were performed using the weighted survey methods in SAS (version 9.4). Weighted values of patient-level observations were generated to produce a nationally representative estimate of the entire US population of hospitalized patients. P-values of <0.05 was considered significant. Univariate analysis of differences between categorical variables was tested using the chi-square test. The mixed-effects survey logistic regression models with weighted analysis were used for the dependent variables, in order to estimate odds ratio (OR) and 95% Confidence Interval for the evaluation of the relationship of chronic periodontitis with cerebral atherosclerosis and cerebrovascular diseases and the relationship of cerebral atherosclerosis with cerebrovascular diseases.
In the multivariable analysis, we included demographics (age, gender, race), patient-level hospitalization variables (admission day, primary payer, admission type, Median Household Income Category), hospitallevel variables (hospital region, teaching versus nonteaching hospital, hospital bed size), comorbidities like diabetes mellitus, hypertension, obesity, hypercholesterolemia, current or past smoker, drug abuse/dependent, alcohol abuse/dependent, AIDS, renal failure (Acute/Chronic/End-stage renal disease or ESRD) and CCI.
For each model, c-index (a measure of goodness of fit for binary outcomes in a logistic regression model) was calculated. All statistical tests used were 2-sided, and p<0.05 was deemed statistically significant. No statistical power calculation was conducted prior to the study.

Data availability
The data that support the findings of this study are publicly available from the Agency for Healthcare Research and Quality's Healthcare Cost and Utilization Project (HCUP) Nationwide Inpatient Sample (NIS). A raw analysis of the data will be however made available from the authors upon request and with permission of HCUP-NIS.

Disease hospitalizations
There was a total of 56,499,768 hospitalizations in the United States from year January 2013 to December 2014 after excluding patients with age <18 years and admissions with missing data for age, gender, and race. Out of 56,499,768 hospitalizations, 7020 (0.01%) had chronic periodontitis.

Demographics, patient and hospital characteristics, and comorbidities
In the US hospitalizations, the prevalence of chronic periodontitis was higher in patients age group 50-64 years (36.18% vs. 23

Regression model derivation
The overall adjusted odds for cerebral atherosclerosis in chronic periodontitis patients among US hospitalizations were 2.48 (95%CI:1.34-4.59, p<0.004), after adjusting for basic demographic with patientlevel variables, comorbidities, CCI, concurrent conditions compared to non-chronic periodontitis patients (

Accuracy of the model
c-statistic was used to validate the accuracy of the regressions. It was 0.772, 0.803, 0.798 for adjusted model 1, 2 and 3 respectively. All models have c-index >0.7, which indicates a good model fit.

Discussion
With increasing prevalence, chronic periodontitis is arising as a major public health concern contributing to the global burden of chronic diseases. According to the CDC, nearly half of the Americans aged 30 or older have periodontitis [3]. Chronic inflammation due to periodontal disease has a causal relation to atherosclerosis mediated by changes in inflammation and the immune response, with subsequent causative relationships to stroke and heart disease [17,18]. Prior observational studies have shown that poor periodontal health status is associated with increased stroke risk. In our large population-based crosssectional study, we aimed to investigate the link between chronic periodontitis and cerebrovascular disease. Further assessment of risk profiles for periodontal disease in adults in the United States shows male sex, current cigarette smoking, and diabetes mellitus as important risk factors for periodontal disease [19].
Chronic periodontitis represents a systemic burden of bacteria, endotoxin, and other bacterial products, which can induce an abundant production of proinflammatory cytokines leading to inflammatory cell proliferation into large arteries and stimulate the hepatic synthesis of clotting factors, thus contributes to atherogenesis and thromboembolic events [1,20,21]. Chronic periodontal disease patients have increased levels of inflammatory cytokines like TNF -alpha, IL-1, and IL-6 which leads to fibrin deposition and atherogenic trigger response [1,22]. These hypotheses of pathogenesis help to establish the association of the periodontal disease with cardiovascular disease, cerebrovascular disease, and atherosclerosis. This may explain our study results of the increased incidence of atherosclerosis in chronic periodontitis.
In this large population-based cross-sectional study we found a direct association between chronic periodontitis with atherosclerosis and in-direct relation to a higher incidence of cerebrovascular disease. US hospitalizations with chronic periodontitis had a higher prevalence in the age group 50-64 years, male, African American, and 0-25th percentile median household income category patients compared to nonchronic periodontitis. Various studies have data on the higher prevalence of chronic periodontitis in African Americans due to poor dental hygiene [23]. In our study, chronic periodontitis was also associated with other comorbidities such as diabetes, hypertension, hyperlipidemia, obesity, drug abuse, alcohol abuse, and tobacco smoking. Interestingly, we found a 2.48 times more risk of cerebral atherosclerosis among chronic periodontitis as compared to no chronic periodontitis. Higher prevalence of AIS, TIA, and HS were noted in chronic periodontitis, but the evidence was not significant (p=0.1560). Higher prevalence of AIS, TIA, and HS are seen in patients with cerebral atherosclerosis as compared to patients without atherosclerosis, with odds of 3.35, 2.40, 1.51 in AIS, TIA, and HS respectively. In our study, we could not establish a direct relationship between cerebrovascular diseases and chronic periodontitis. Therefore, chronic periodontitis does not directly increase the risk of cerebrovascular diseases but increases the risk of cerebral atherosclerosis which is associated with increased risk of cerebrovascular diseases.
Several observational meta-analysis studies have shown that chronic periodontitis and poor periodontal health status is associated with an increased risk for stroke and cardiovascular diseases [24,25]. A large population-based, nationwide study in Taiwan identified the periodontal disease as an important risk factor for ischemic stroke incidence and showed that periodontal treatment lowered the risk of stroke, significantly among young adults [26]. Grau et al. carried out a case-control study in the German population, in which increasing severity of periodontitis was associated with an increased risk of ischemic stroke after adjustment for age, gender, number of teeth, and other covariables [27]. A more recent meta-analysis of two cohort studies found that periodontal disease increased the risk of ischemic strokes by 1.6-fold [5].
It is possible that an increased risk of thrombotic stroke may be secondary to atherothrombosis in the cervicocerebral vasculature and cardioembolic stroke association may be because of coronary artery disease or atrial fibrillation related to periodontal disease-induced inflammation. A recent study by Sen et al. cohort of the ARIC database was studied over a period of 15 years which demonstrated an increased hazard of cardioembolic and thrombotic stroke in chronic periodontitis. The study also shows regular dental care use continued to be associated with lower rates of ischemic stroke [28].
Our study analysis showed no direct linkage between cerebrovascular diseases and chronic periodontitis, but chronic periodontitis was significantly associated with an increased prevalence of cerebrovascular diseases. This contradictory finding may have arisen as periodontitis is usually managed on an outpatient basis, so inpatient data of chronic periodontitis prevalence might be underrepresented. There is a need to be more vigilant in diagnosing chronic periodontitis on both inpatient and outpatient basis. Early detection of chronic periodontitis, treatment, and stressing the need for dental hygiene can be emphasized which ultimately will lead to a reduction in the risk of cerebrovascular events.
We strongly recommend that chronic periodontitis should be identified as an important risk factor for cerebral atherosclerosis and a possible risk for cerebrovascular diseases (AIS, TIA, and HS). Poor oral hygiene which contributes to periodontal disease is a modifiable risk factor. Evidence from randomized controlled trials has established that intensive periodontal treatment improves systemic inflammation, high blood pressure, lipid profile, and endothelial dysfunction [29,30], further reducing the risk of atherosclerotic disease. Frequent tooth brushing and regular dental care will potentially reduce the risk of cerebrovascular diseases.

Strength and Limitations
To the best of our knowledge, this would be the first US-based large population cross-sectional study to report the relationship between chronic periodontitis and cerebral atherosclerosis. NIS Data represent the largest inpatient database. This study does have some limitations. We were not able to establish a direct causal relationship between chronic periodontitis and cerebrovascular disease with good statistical power. But this study successfully puts forward an indirect relationship between chronic periodontitis and cerebrovascular diseases. Diagnosis of chronic periodontitis in the NIS database is the physiciandocumented diagnosis, hence susceptible to coding errors, and with chronic periodontitis being usually managed in an outpatient setting, might have been underdiagnosed as a hospital problem. We were not able to quantify the severity of chronic periodontitis.

Conclusions
Our study found a significant association of cerebral atherosclerosis with chronic periodontitis. Furthermore, we could not establish the directly associated risk of chronic periodontitis with cerebrovascular disease, but it increases the burden of cerebrovascular diseases through evidently increasing the risk of cerebral atherosclerosis. Early identification of chronic periodontitis and atherosclerotic risk factors helps to mitigate the risk of cerebrovascular diseases.

Additional Information Disclosures
Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue.

Conflicts of interest:
In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.