Simultaneous Clinical Presentation of Renal Cell Carcinoma and Immunoglobulin Light Chain Amyloidosis

A 77-year-old female was admitted to the hospital for an evaluation of congestive heart failure. She gave a history of progressive peripheral edema over eight to 10 months, extending up to the knees bilaterally. Admitting creatinine was 148 mmol/L, serum albumin was 15g/L, and urine protein on quantification was 9.09 g/day. Her immunoglobulin G (IgG) level was 18.4g/L and serum-free kappa level was 92.3 mg/L. The immunofixation of urine revealed monoclonal IgG kappa (1.97 g/d). Her kidney biopsy subsequently confirmed the diagnosis of immunoglobulin light chain (AL) amyloidosis. During the course of investigations, it was incidentally noted that she had a mass on her right kidney, which on biopsy was identified as renal cell carcinoma (RCC). This case deals with the rare situation of AL amyloidosis existing with a solid organ carcinoma and the therapeutic dilemma of treating two unrelated conditions involving the kidneys.


Introduction
Amyloidosis in the context of RCC usually presents as amyloid A (AA) amyloidosis (systemic amyloidosis) in up to 5% of patients and reflects a chronic inflammatory response, as the amyloid fibrils are composed of fragments of the acute phase reactant serum amyloid A protein [1][2]. This is the first reported case of immunoglobulin light chain AL (kappa) amyloidosis with nephrotic syndrome occurring in conjunction with renal cell carcinoma.

Case Presentation
A 77-year-old Caucasian female was admitted to the hospital for an evaluation of congestive heart failure. She gave a history of progressive peripheral edema over eight months, extending up to the knees bilaterally. She felt weak, exhausted, and had lost her appetite. She denied any orthopnoea or paroxysmal nocturnal dyspnea. She conceded to a long-standing history of hypertension, chronic atrial fibrillation, and hypothyroidism but denied any history of diabetes. Initial investigations, which included a urine analysis, revealed the presence of protein and no evidence of blood. Her admitting creatinine was 148 (micromol/l); serum albumin 15 g/L, and ACR 1025 mg/mmoL (normal <2.8 mg/mmoL). She had a normal white cell count and platelets and her hemoglobin was 115 g/L.
She subsequently underwent a confirmatory kidney biopsy. On light microscopy, there were 21 glomeruli, four of which were sclerosed. Congo red positive staining was identified. Electron microscopy revealed haphazardly arranged thin fibrils in the mesangium and glomerular basement membrane ( Figure 1). Upon further interrogation of the biopsy with liquid chromatography tandem-mass spectrometry (LC MS/MS), a peptide profile consistent with AL (kappa) type amyloid deposit was identified. These changes were histologically consistent with a diagnosis of AL amyloidosis. Clinically, she was diagnosed with AL amyloidosis with nephrotic syndrome. During the biopsy, it was noted that there was a solid mass in the left kidney. We investigated it further with computed tomography (CT) of the abdomen, which identified a 2.1 x 1.9 cm cystic lesion (23 Hounsfield units), but in the absence of contrast, the radiologist couldn't make a further determination. Subsequent magnetic resonance imaging (MRI) revealed a 2.3 X 2.2 cm lesion in the upper pole of the left kidney suspicious of a tumor ( Figure 2) and the presence of a single retroperitoneal lymph node, which was biopsied and identified to be benign with no evidence of amyloid. The biopsy of the renal mass was consistent with grade 1 renal cell carcinoma. Her inpatient stay was further complicated by progressive ascites, which required repeated paracentesis. The presence of two concurrent, yet unrelated, diseases involving the kidney led to a therapeutic dilemma. After a discussion with the oncologist, it was decided that RCC was a slow-growing carcinoma and that surgical treatment could be offered upon stabilization of her underlying amyloidosis. She was initiated on 28-day cycles of 1.2 mg/m 2 of bortezomib, 500 mg of cyclophosphamide, and 20 mg of dexamethasone. Unfortunately, despite the initiation of therapy, her proteinuria worsened, leading to repetitive drainage of her ascites and, later, she became neutropenic and septic. The family later withdrew active care, and she was transitioned to comfort measures and passed away peacefully three days later.

FIGURE 2: MRI of the kidneys
The highlighted renal mass was biopsied and proven to be renal cell carcinoma.

Discussion
Immunoglobulin light chain (AL) amyloidosis is a protein misfolding disease characterized by the extracellular deposition of an amyloidogenic monoclonal light chain [3]. The amyloidogenic light chains (LCs) are produced by B-cell clones, most frequently a plasma cell. This process results in progressive organ injury that can lead to death in months to years. The kidney along with the heart remains the most common targeted organ. Despite recent advances in pharmacotherapy, both renal and overall prognosis remain poor.
Okamoto et al. recently published a case of AL amyloidosis existing concurrently with lung adenocarcinoma [4]. There are no other recent case reports or series that have documented the association of AL amyloidosis with solid organ tumors. The majority of the association of renal cell carcinoma with amyloid is AA amyloid and reflects a chronic inflammatory response as the amyloid fibrils are composed of fragments of the acute phase reactant serum amyloid A protein.
In our patient, the presence of AL amyloidosis and RCC lead to the involvement of multiple care providers and the decision was based on her poor premorbid state of health, frailty, the lack of aggressiveness of the underlying solid organ neoplasm, and intractable ascites as a consequence of hypoalbuminemia from nephrotic syndrome secondary to amyloidosis.

Conclusions
To our knowledge, this is the first reported case of concurrent RCC with AL amyloidosis. The previous reports in the literature are of AA amyloidosis. The treatment decision depends on the relative aggressiveness of the underlying disease states and the premorbid state of the individual. AL amyloidosis is associated with a high rate of mortality and the outcomes, irrespective of the treatment strategy, remain poor.

Additional Information Disclosures
Human subjects: Consent was obtained by all participants in this study.

Conflicts of interest:
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